Review



eclipse ts2 fl inverted microscope  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Nikon eclipse ts2 fl inverted microscope
    Eclipse Ts2 Fl Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/Objectives/pmc13022049-152-6-5
    Average 99 stars, based on 59909 article reviews
    eclipse ts2 fl inverted microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.

    Imaging:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.

    Software:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.

    Expressing:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.

    Staining:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.

    Immunolabeling:

    Article Title: Multifunctional Tri-layer Wound Dressing containing ZnO nanoparticles and IGF-1 as an Efficient Biomaterial for Healing of Full Thickness Skin Injuries
    Article Snippet: Slides were visualized under a Nikon Eclipse Ts2-FL or Olympus BX41 microscope equipped with a Logene LG1000 digital camera system for detection and analysis of IL-6, TNF-α, CD31, CD34, and VEGF expression.

    Article Title: Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway.
    Article Snippet: factors and phosphorylated MITF.. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells.. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSHinduced B16F1 cells.

    Article Title: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1.
    Article Snippet: After 3 days, phase contrast images were acquired using a 20x (NA, 0.4) dry objective (#MRP46202, Nikon, Miyato, Japan) on an Eclipse Ts2-FL (Nikon, Miyato, Japan) microscope equipped with a 1920×1200 pixel monochromatic camera (#IS-DMK33UX174, Nikon, Miyato, Japan).

    Article Title: CONDENSATES OF SYNAPTIC VESICLES AND SYNAPSIN ARE MOLECULAR BEACONS FOR ACTIN SEQUESTERING AND POLYMERIZATION
    Article Snippet: After measurements, all conditions were imaged using a Nikon Eclipse TS2-FL microscope, capturing widefield signal and GFP-epifluorescence.

    Article Title: 2,3-Diphosphoglyceric Acid Alleviating Hypoxic-Ischemic Brain Damage through p38 MAPK Modulation
    Article Snippet: Finally, the stained cells were visualized and imaged using a Nikon Eclipse Ts2-FL microscope (Nikon, Tokyo, Japan).

    Article Title: Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death
    Article Snippet: Images were acquired randomly in each well with a Nikon Eclipse Ts2-FL microscope (Nikon, Champigny-sur-Marne, France) equipped with a Nikon DS-Fi3 camera interfaced with the NIS-Elements imaging software (Nikon, Melville, USA), using the following parameters: excitation wavelength = 485 nm/emission wavelength = 530 ± 30 nm (calcein-AM channel); excitation wavelength = 530 nm/emission wavelength = LP 590 nm (propidium iodide channel).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, the cell nuclei were stained with DAPI (D9542, Sigma-Aldrich) and visualized using a Nikon Eclipse Ts2-FL microscope (Nikon, Japan).

    Article Title: Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1
    Article Snippet: Finally, differentiated muscle cells were observed using a Nikon Eclipse Ts2-FL microscope and quantified using NIS-Element imaging software.



    Similar Products

    99
    Nikon eclipse ts2 fl inverted microscope
    Eclipse Ts2 Fl Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/Objectives/pmc13022049-152-6-5
    Average 99 stars, based on 1 article reviews
    eclipse ts2 fl inverted microscope - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Nikon eclipse ts2-fl microscope
    Eclipse Ts2 Fl Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/pmc12209937-46-9-13
    Average 90 stars, based on 1 article reviews
    eclipse ts2-fl microscope - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    99
    Nikon eclipse ts2 fl microscope
    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse <t>Ts2</t> Fl <t>microscope</t> using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.
    Eclipse Ts2 Fl Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/Objectives/pmc12616069-190-7-6
    Average 99 stars, based on 1 article reviews
    eclipse ts2 fl microscope - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Nikon fluorescence microscope nikon eclipse ts2-fl
    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse <t>Ts2</t> Fl <t>microscope</t> using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.
    Fluorescence Microscope Nikon Eclipse Ts2 Fl, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/bio_rxiv__2025__07__10__664241-107-8-10
    Average 90 stars, based on 1 article reviews
    fluorescence microscope nikon eclipse ts2-fl - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Staining, Microscopy

    KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Shear, Labeling, Fluorescence, Microscopy, Binding Assay, Flow Cytometry